Heterologous expression of fungal cytochromes P450 (CYP5136A1 and CYP5136A3) from the white-rot basidiomycete Phanerochaete chrysosporium: Functionalization with cytochrome b5 in Escherichia coli
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- 作者：Mayumi Hatakeyama m_hatakeyama@agr.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Takuya Kitaoka tkitaoka@agr.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Hirofumi Ichinose ; ichinose@agr.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：P450 ; cytochrome P450 monooxygenase ; NTD ; N-terminal domain ; PR ; proline-rich ; CPR ; cytochrome P450 oxidoreductase ; Cyt-b5 ; cytochrome b5 ; CB5R ; NADH-dependent cytochrome b5 reductase ; HEPES ; [4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid ; EDTA ; ethylenediaminetetraacetic acid ; IPTG ; isopropyl-1-thio-β-d-galactopyranoside ; CHAPS ; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：89
- 期：Complete
- 页码：7-14
- 全文大小：806 K
文摘
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Heterologous expression systems for CYP5136A1 and CYP5136A3 were constructed.
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Expression levels were dramatically improved by N-terminal sequence modifications.
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An alternative electron transfer system was demonstrated for CYP5136A1.