Differential expression of cysteine peptidase genes in the inner integument and endosperm of developing seeds of Jatropha curcas L. (Euphorbiaceae)
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- 作者：Ant么nio J. Rocha ; Emanoella L. Soares ; Jos茅 H. Costa ; Washington L.G. Costa ; Arlete A. Soares ; F谩bio C.S. Nogueira ; Gilberto B. Domont ; Francisco A.P. Campos
- 关键词：BLAST ; basic local alignment search tool ; cDNA ; complementary DNA ; EST ; expressed sequence tag ; KDEL-CysEP ; KDEL-tailed cysteine peptidase ; nr ; non redundant ; SRA ; sequence read archive ; TSA ; transcriptome shotgun assembly ; VPE ; Vacuolar processing enzyme ; WGS ; whole-genome shotgun
- 刊名：Plant Science
- 出版年：December, 2013
- 年：2013
- 卷：213
- 期：Complete
- 页码：30-37
- 全文大小：2111 K
文摘
In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-尾VPE, Jc-纬VPE and Jc-未VPE) and determined the expression patterns of these genes by RT-qPCR in integument and cellular endosperm of seeds collected at seven different developmental stages. We were able to demonstrate that the expression of Jc-CysEP1, Jc-CysEP2, Jc-尾VPE and Jc-纬VPE proceeded rapidly from Stage IV, with Jc-CysEP2 displaying the highest relative expression; expression of Jc-未VPE could not be detected in any of the tissues/developmental stages analyzed. Additionally, we showed that the expression pattern of these peptidases correlates with anatomical changes in integument and cellular endosperm, thus suggesting a role for both classes of peptidases in PCD and in protein processing, both of which occur simultaneously in each of these tissues.