Hydrogen sulfide is produced by cystathionine ¦Ã-lyase at the steady-state low intracellular Ca²⁺ concentrations

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• 作者：Yoshinori Mikami^a ; Norihiro Shibuya^a ; Yuki Ogasawara^b ; Hideo Kimura^a ; ^{kimura@ncnp.go.jp}
• 关键词：Hydrogen sulfide ; Cystathionine ¦Ã-lyase ; Calcium ; Pyridoxal 5&prime ; -phosphate
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：8 February, 2013
• 年：2013
• 卷：431
• 期：2
• 页码：131-135
• 全文大小：330 K

文摘

Hydrogen sulfide (H₂S) is recognized as a physiologic mediator produced in a variety of tissues. It is produced by three enzymes, cystathionine ¦Â-synthase (CBS), cystathionine ¦Ã-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3MST). However, the regulation of H₂S production by CSE has not well been understood. Here we show that H₂S producing activity of CSE is regulated by intracellular Ca²⁺ concentrations. In the presence of pyridoxal 5¡ä-phosphate (PLP) CSE efficiently produces H₂S at the steady-state low Ca²⁺ concentrations but is suppressed at high Ca²⁺ concentrations. In the absence of PLP H₂S production maintains the suppressed levels at high Ca²⁺ concentrations and decreased further at low Ca²⁺ concentrations. These observations suggest that CSE produces H₂S at the steady-state in cells and that the production is suppressed when the intracellular Ca²⁺ concentrations are increased.