A fusion protein system for the recombinant production of short disulfide bond rich cystine knot peptides using barnase as a purification handle

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• 作者：Schmoldt ; Hans-Ulrich ; Wentzel ; Alexander ; Becker ; Stefan ; Kolmar ; Harald
• 关键词：Cystine knot peptide ; Fusion protein ; Barnase ; Escherichia coli expression
• 刊名：Protein Expression and Purification
• 出版年：2005
• 出版时间：January, 2005
• 年：2005
• 卷：39
• 期：1
• 页码：82-89
• 全文大小：665 K

文摘

The inhibitor cystine knot (ICK) structural motif has been found in several small proteins and peptides from plants, insects, marine molluscs, and also in human. It is defined by a triple ??-sheet that is held together by three intramolecular disulfide bonds built by six conserved cysteine residues that generate a highly rigid and stable fold. We describe a procedure for the production of ICK peptides with correct disulfide bond connectivities via expression in Escherichia coli as fusion proteins with an enzymatically inactive variant of the Bacillus amyloliquefaciens RNAse barnase. Barnase directs the fused peptide to the culture medium and the fusion protein can be isolated by combined cation exchange/reverse-phase chromatography. The ICK peptides are released from the barnase expression and purification handle either by cyanogen bromide or by protease cleavage to give pure and correctly folded cystine knot peptides.