Metalloprotein-based MRI probes

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• 作者：Yuri Matsumoto^a ; Alan Jasanoff^a ; ^b ; ^c ; ^{jasanoff@mit.edu}
• 关键词：BBB ; blood&ndash ; brain barrier ; BOLD ; blood oxygen level dependent ; CNS ; central nervous system ; BM3h ; cytochrome P450 BM3 heme domain ; EC50 ; effective concentration midpoint ; T1e ; electronic relaxation time ; S ; electron spin number ; Ft ; ferritin ; fMRI ; f
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：17 April, 2013
• 年：2013
• 卷：587
• 期：8
• 页码：1021-1029
• 全文大小：718 K

文摘

Metalloproteins have long been recognized as key determinants of endogenous contrast in magnetic resonance imaging (MRI) of biological subjects. More recently, both natural and engineered metalloproteins have been harnessed as biotechnological tools to probe gene expression, enzyme activity, and analyte concentrations by MRI. Metalloprotein MRI probes are paramagnetic and function by analogous mechanisms to conventional gadolinium or iron oxide-based MRI contrast agents. Compared with synthetic agents, metalloproteins typically offer worse sensitivity, but the possibilities of using protein engineering and targeted gene expression approaches in conjunction with metalloprotein contrast agents are powerful and sometimes definitive strengths. This review summarizes theoretical and practical aspects of metalloprotein-based contrast agents, and discusses progress in the exploitation of these proteins for molecular imaging applications.