Masks in imaging flow cytometry

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• 作者：Venina Dominical ; Leigh Samsel ; J. Philip McCoy Jr. ; ^{mccoyj@nhlbi.nih.gov}
• 关键词：Imaging flow cytometry ; Mask ; Features ; Data analysis ; ImageStream
• 刊名：Methods
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：9-17
• 全文大小：2045 K
• 卷排序：112

文摘

Data analysis in imaging flow cytometry incorporates elements of flow cytometry together with other aspects of morphological analysis of images. A crucial early step in this analysis is the creation of a mask to distinguish the portion of the image upon which further examination of specified features can be performed. Default masks are provided by the manufacturer of the imaging flow cytometer but additional custom masks can be created by the individual user for specific applications. Flawed or inaccurate masks can have a substantial negative impact on the overall analysis of a sample, thus great care must be taken to ensure the accuracy of masks. Here we discuss various types of masks and cite examples of their use. Furthermore we provide our insight for how to approach selecting and assessing the optimal mask for a specific analysis.