Senescence-related functional nuclear barrier by down-regulation of nucleo-cytoplasmic trafficking gene expression
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- 作者：Sung Young Kim ; Sung Jin Ryu ; Hong Ju Ahn ; Hae Ri Choi ; Hyun Tae Kang ; Sang Chul Park
- 关键词：Aging ; Senescence ; Nucleo-cytoplasmic trafficking ; Nuclear pore complex
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2010
- 出版时间：1 January 2010
- 年：2010
- 卷：391
- 期：1
- 页码：28-32
- 全文大小：738 K
文摘
One of the characteristic natures of senescent cells is the hypo- or irresponsiveness not only to growth factors but also to apoptotic stress. In the present study, we confirmed the inhibition of nuclear translocation of activated p-ERK1/2 and NF-kB p50 in response to growth stimuli or LPS in the senescent human diploid fibroblasts. In order to elucidate the underlying mechanism for the senescence-associated hypo-responsiveness, we carried out the comparison study for gene expression profiles through microarray analysis. In consequence, we observed the vast reduction in expression of nucleo-cytoplasmic trafficking genes in senescent cells, when compared with those in young cells. Expression levels of several nucleoporins, karyopherin α, karyopherin β, Ran, and Ran-regulating factors were confirmed to be down-regulated in senescent HDFs by using RT-PCR and Western blot methods. Taken together, these data suggest the operation of certain senescence-associated functional nuclear barriers by down-regulation of the nucleo-cytoplasmic trafficking genes in the senescent cells.