The Use of Sequential VEGF- and BMP2-Releasing Biodegradable Scaffolds in Rabbit Mandibular Defects

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• 作者：Nilü ; fer Ç ; akır-Ö ; zkan ; DDS^&lowast ; ; ^{nfozkan@hotmail.com} ; Sinan Eğri ; DDS^&dagger ; ; Esengü ; l Bekar ; DDS^&Dagger ; ; B. Zuhal Altunkaynak ; DDS ; MD^§ ; ; Yonca Betil Kabak ; DVM^‖ ; Elfide Gizem Kıvrak^¶ ;
• 刊名：Journal of Oral and Maxillofacial Surgery
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：75
• 期：1
• 页码：221.e1-221.e14
• 全文大小：10016 K
• 卷排序：75

文摘

Promising developments have materialized in reconstructive surgical procedures with the applications of tissue engineering. In our study, we used tissue scaffolds fabricated from polylactic acid-polyethylene glycol (PLLA-PEG) copolymers to ensure different release rates of selective growth factors recombinant human bone morphogenetic protein 2 [rhBMP-2] and vascular endothelial growth factor (rhVEGF165) in the repair of mandibular bone defects.Materials and MethodsIn our experimental study, 54 New Zealand rabbits were used. The rabbits were separated into 4 groups: group I (control group), PLLA-PEG scaffold only; group II, PLLA-PEG scaffold plus rhBMP-2 application; group III, PLLA-PEG scaffold plus VEGF165 application; and group IV, PLLA-PEG scaffold plus rhBMP-2 and VEGF165 applications. The rabbits were killed at 4 and 8 weeks postoperatively, and histopathologic and immunohistochemical assessments were performed.ResultsThe greatest bone volume was observed in rhBMP-2–containing groups, the greatest vessel volume was observed in VEGF165-containing groups; however, the scaffold containing rhBMP-2 and VEGF165 provided the best outcomes in conjunction with increased remodeling of the new bone.ConclusionsThe use of polymer tissue scaffolds that release rhVEGF165 and rhBMP-2 in coordination and mimic the natural healing process in the regeneration of especially complex tissues, such as bone, is a promising treatment alternative in the field of reconstructive surgery.