Characteristics and frequency of DEL phenotype detected by indirect antiglobulin test in Dalmatia county of Croatia

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• 作者：Slavica Dajak^a ; ^{slavica.dajak@kbsplit.hr" class="auth_mail} ; Jelena Lukacevic Krstic^a ; Gü ; nther Kö ; rmö ; czi^b ; Vesna Dogic^c ; Vedrana Burilovic^a
• 关键词：Weak D variants ; Indirect antiglobulin test ; Genotyping RHD
• 刊名：Transfusion and Apheresis Science
• 出版年：April 2014
• 年：2014
• 卷：50
• 期：2
• 页码：210-213
• 全文大小：244 K

文摘

D variants, collectively called DEL, express trace amounts of D antigen which is considered to be serologically detectable only by adsorption–elution techniques.

We detected six cases of DEL phenotype by indirect antiglobulin test, in Dalmatia County of Croatia by routine serological testing of D antigen of new blood donors. RH genotyping found that all six donors carry allele RHD(M295I), RH genotype CcDdee. D antigen densities of D variants were very low, between 26 and 44 D antigens per red blood cell. The frequency of D variants detected by IAT allele RHD(M295I) was 1:272 in D negative donors. Obviously, DEL phenotype is more common in some parts of European population than initially thought.

In conclusion, our routine serological testing of D antigen can detect extremely weak D antigen, even RBCs with DEL phenotype and antigen density as low as 26 D antigens per RBC.