A suicidal DNA vaccine expressing the fusion protein of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in mice
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- 作者：Yong Wanga ; e ; Xiaolin Yuea ; Hongyan Jinb ; Guangqing Liuc ; Ling Pana ; Guijun Wanga ; Hao Guoa ; Gang Lib ; gli358@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Yongdong Lid ; liyd@nbcdc.org.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：BHK-21 cell ; baby hamster kidney ; CPE ; cytopathic effect ; DAB ; 3 ; 3&prime ; -diaminobenzidine tetrahydrochloride ; DIVA ; differentiating infected from vaccinated animals ; DMEM ; Dulbecco's modified Eagle's medium ; F protein ; fusion protein ; GMT ; geometric mean titer ; H ; hemagglutinin ; HRP ; horseradish peroxidase ; IFA ; indirect immunofluorescence assay ; kDa ; kilo Dalton ; Nig 75/1 ; Nigeria 75/1 ; OIE ; World Organization for Animal Health ; PPR ; peste des petits ruminants ; PPRV ; peste des petits ruminants
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：225
- 期：Complete
- 页码：35-40
- 全文大小：1031 K
文摘
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A suicidal DNA vaccine against peste des petits ruminants virus (PPRV) was developed.
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The suicidal DNA vaccine could express Fusion protein in transfected BHK-21 cells.
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Specific humoral and cell-mediated immune responses were induced in immunized mice.
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The results provide a new strategy to the development of vaccines against PPRV.