20-hydroxyecdysone mediates fat body arylphorin regulation during development of rice moth, Corcyra cephalonica
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- 作者：V. VenkatRao ; R.K. Chaitanya ; A. Dutta-Gupta ; apdgsl@uohyd.ernet.in" class="auth_mail" title="E-mail the corresponding author ; aparnaduttagupta@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：° ; C ; Degree centigrade/Celsius ; 20E ; 20-Hydroxyecdysone ; cDNA ; Complementary DNA ; DNA ; Deoxyribonucleic acid ; EDTA ; Ethylene diamino tetra acetic acid ; ELI ; Early-last instar larvae ; EMSA ; Electrophoretic mobility shift assay ; JH ; Juvenile hormone ; kDa ; Kilo dalton ; LLI ; Late-last instar larvae ; mg ; Milligram ; MLI ; Mid-last instar larvae ; mM ; Millimolar ; mRNA ; Messenger ribonucleic acid ; nM ; Nanomolar ; PAGE ; Polyacrylamide gel electrophoresis ; PBS ; Phosphate buffered saline ; PCR ; Polymerase c
- 刊名：Gene
- 出版年：2016
- 出版时间：10 January 2016
- 年：2016
- 卷：575
- 期：2
- 页码：747-754
- 全文大小：1582 K
文摘
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Expression of arylphorin begins in the early instar and reaches its maximum level at last instar larval stage.
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20E causes the upregulation of arylphorin both at the mRNA and protein levels.
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Deletion analysis revealed that − 258 upstream region is sufficient to drive the transcription of arylphorin.
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A 19 bp functional Ecdysone Responsive Element in the 5′ upstream region of arylphorin has been identified.