Embelin inhibits proliferation, induces apoptosis and alters gene expression profiles in breast cancer cells

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• 作者：Priyank Shah ; Ransford Djisam ; Hamidah Damulira ; Alice Aganze ; Michael Danquah ; ^{mdanquah@csu.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Embelin ; Breast cancer ; Gene expression profiling ; RT2 Profiler PCR Array ; Apoptosis
• 刊名：Pharmacological Reports
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：68
• 期：3
• 页码：638-644
• 全文大小：1156 K

文摘

To investigate effect of embelin on proliferation, apoptosis and gene expression profile changes in breast cancer cells.

Methods

Cell viability was determined by MTT assay and apoptosis assayed using flow cytometry. Differential expression of 84 genes commonly involved in breast cancer carcinogenesis was assessed by real-time PCR using the Human Breast Cancer RT² Profiler PCR Array.

Results

MCF-7 and MDA-MB-231 cells were treated with embelin (0–25 μM) for 24 and 96 h. Embelin exhibited time and dose dependence in both cell lines and was more potent in inhibiting MDA-MB-231 cell proliferation compared to MCF-7 cells. IC₅₀ for embelin in MDA-MB-231 cells was ∼4.45 μM and 3.28 μM at 24 h and 96 h, respectively. In contrast, IC₅₀ for embelin in MCF-7 cells was ∼6.04 μM and 4.51 μM at 24 h and 96 h, respectively. Embelin (50 μM) induced apoptosis and activated caspase 3 activity in both cell lines when exposed for 72 h. Treatment of MDA-MB-231 cells with embelin (10 μM) for 24 h resulted in significant differential expression of 27 genes commonly involved in breast cancer carcinogenesis.

Conclusions

Our findings show that embelin inhibits cell proliferation, induces apoptosis and alters expression of breast cancer focused genes in MCF-7 and MDA-MB-231 cells. Based on RT²-PCR array analysis, embelin down-regulated expression of pivotal oncogenes. This knowledge could be beneficial in the development of effective embelin-based therapies for treating breast cancer.