Identification of eleven novel HLA alleles by sequence based typing (SBT): A∿2:557, A∿1:01:64, B∿0:299, B∿3:37, C∿1:98 N, C∿2:14:02, C∿4:195, C∿8:110, C∿8:111, DRB1∿3:110, DRB∿3:184

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• 作者：Brandt Moore ; Weicheng Zhao ; Dana Willis ; Asdrubal Lopez ; Siqi Liao ; Titus Barnes ; Hai-Ho Hoang ; Vinh Ngo ; William Hamm ; Vickie Mai ; Qing Wang ; Edward Guerrero ; Kai Cao
• 刊名：Human Immunology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：76
• 期：supp_S
• 页码：102
• 全文大小：405 K

文摘

Accurate HLA typing and matched donor selection is essential for graft outcome in patients receiving a stem cell transplant. Misidentification of new alleles can result in graft failure or acute graft vs. host disease. This study identifies eleven novel HLA alleles at class I and class II loci.

Method

Unusual or potentially novel HLA alleles were detected during sequence specific oligonucleotide (SSO) intermediate resolution typing. Characterization of novel alleles was performed by sequence based typing (SBT) using group specific and locus specific primers.

Results

Eleven novel HLA sequences were identified and characterized in 14 individuals, including HLA-A∗11:01:64 in a patient and a haploidentical sibling, C∗04:195 in a patient and a fully matched sibling, and DRB1∗03:110 in two haploidentical sibling donors. Nine of the eleven novel HLA alleles resulted in an amino acid substitution. The null allele C∗01:98 N resulted in a premature stop codon in exon 2. Six out of the eleven novel alleles have nucleotide/amino acid changes in exon 4 and the rest in exon 2. Table 1 shows the differences of nucleotides and amino acids between these novel alleles and their most homologous allele and the location of the changes.

Discussion

Identification and characterization of novel HLA alleles is important for the selection of appropriate stem cell transplant donors aimed at improving graft outcome and patient survival.