Poor recognition of HIV-1 Nef protein by CD8 T cells from HIV-1-infected children: Impact of age

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• 作者：Florence Buseyne ; Daniel Scott-Algara ; Bé ; atrice Corre ; Franç ; oise Porrot ; Elizabeth Monchatre ; Nassima Bellal ; Marianne Burgard ; Christine Rouzioux ; Sté ; phane Blanche and Yves Riviè ; r
• 关键词：HIV ; HIV perinatal infection ; Elispot ; CTL ; CD8 T cell ; Children
• 刊名：Virology
• 出版年：2006
• 出版时间：25 October 2006
• 年：2006
• 卷：354
• 期：2
• 页码：271-279
• 全文大小：552 K

文摘

Recognition of various HIV proteins by CD8 T cells from HIV-infected children was determined by two functional assays. First, using an Elispot assay, we show that 80 % of patients recognized Gag, 77 % recognized Pol, 61 % recognized Env, 44 % recognized Nef and 29 % recognized Vif. Frequencies of Gag-, Pol-, and Env-specific IFN-γ producing CD8 T cells were higher than frequencies of Nef and Vif-specific CD8 T cells. The poor recognition of Nef by ex vivo CD8 T cells was confirmed by CTL assays performed in HAART naïve children: 25 % of children had positive response against Nef versus 44, 63 and 62 % for Env, Gag, and Pol, respectively. Memory Gag-specific CTL were positively correlated with age, whereas Nef-specific CTL were negatively correlated with age. The poor Nef-specific CD8 T cell response in HIV-infected children contrasts with dominance of Nef-specific responses in infected adults.