Phenotype and function of myeloid dendritic cells derived from African green monkey blood monocytes
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- 作者：Lorenzo Mortara ; Mickaë ; l J.-Y. Ploquin ; Abdourahmane Faye ; Daniel Scott-Algara ; Bruno Vaslin ; Cé ; cile Butor ; Anne Hosmalin ; Franç ; oise Barré ; -Sinoussi ; Ousmane M. Diop and Michaela C.
- 关键词：MDDC ; Non-human primate ; Semi-mature ; IL-10 ; IL-12
- 刊名：Journal of Immunological Methods
- 出版年：2006
- 出版时间：20 January 2006
- 年：2006
- 卷：308
- 期：1-2
- 页码：138-155
- 全文大小：418 K
文摘
Myeloid dendritic cells probably play an important role in the immune response against HIV and SIV, and in the enhancement of CD4+ T cell infection. Here, we have investigated phenotypic and functional features of myeloid monocyte-derived DC (MDDC) from African green monkeys (AGMs). AGMs are natural hosts of SIV and exhibit no signs of abnormal T cell activation despite high SIV plasma viremia. We identified mAbs that cross-react specifically with homologous molecules expressed on AGM DC. We adapted a protocol to derive AGM MDDC by culture in the presence of GM-CSF and IL-4. The differentiated cells possessed a typical dendritic morphology and the majority were CD11c+ DC-SIGN+. AGM MDDC displayed a high expression of typical maturation markers, such as CD83, CD86 and DC-LAMP, and moderate immunostimulatory capacity, suggesting that the cells were in a semi-mature state. Stimulation resulted in further maturation, as shown by up-regulation of CD80 and decrease of endocytosis ability. However, neither increase of HLA-DR or CD40 expression nor enhanced immunostimulatory capacity was observed. The latter was associated with a low pro-inflammatory cytokine production during mixed lymphocyte reactions and a cytokine balance in favour of IL-10 in contrast to human MDDC. This is the first characterization of AGM MDDC. The tools described here are a crucial step for future studies in vivo or in vitro on the function of myeloid DC using the AGM animal model.