An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

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• 作者：Ka-Ho Leung^aAuthor Vitae ; Hong-Zhang He^aAuthor Vitae ; Daniel Shiu-Hin Chan^aAuthor Vitae ; Wai-Chung Fu^aAuthor Vitae ; Chung-Hang Leung^bAuthor Vitae ; Dik-Lung Ma^a ; ^{edmondma@hkbu.edu.hk}Author Vitae
• 关键词：Kanamycin ; Luminescent ; Aptamer ; Platinum(II) complex ; Switch-on probe
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：177
• 期：Complete
• 页码：487-492
• 全文大小：501 K

文摘

We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution.