Comparison of pectin-degrading fungal communities in temperate forests using glycosyl hydrolase family 28 pectinase primers targeting Ascomycete fungi
详细信息 查看全文
- 作者：Matthew D. Gacura ; mgacura@kent.edu" class="auth_mail" title="E-mail the corresponding author ; Daniel D. Sprockett1 ; Bess Heidenreich ; Christopher B. Blackwood
- 关键词：Fungi ; PCR primers ; Forest litter ; Environmental samples ; Functional gene ; Endopolygalacturonase
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：108-113
- 全文大小：365 K
文摘
- •
Primers were designed for amplification of glycosyl hydrolase family 28 (GH28) genes found in Ascomycetes.
- •
Primers successfully amplified GH28 genes in pure cultures and senesced leaves.
- •
Ecosystem type explained more variation in taxonomic profiles than in GH28 distribution.
- •
Primers will be useful in screening fungal cultures and in environmental assays.