Biological and biochemical characterization of new basic phospholipase A₂ BmTX-I isolated from Bothrops moojeni snake venom

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• 作者：Andrana K. Calgarotto ; Daniela C.S. Damico ; L.A. Ponce-Soto ; Paulo A. Baldasso ; Saulo L. Da Silva ; Gustavo H.M.F Souza ; Marcos N. Eberlin ; Sergio Marangoni
• 关键词：Characterization ; Asp49 phospholipase A2 ; Snake venom ; Neuromuscular blockade ; Edema-forming activity ; Myonecrosis and interleukin-6 response
• 刊名：Toxicon
• 出版年：2008
• 出版时间：15 June 2008
• 年：2008
• 卷：51
• 期：8
• 页码：1509-1519
• 全文大小：504 K

文摘

BmTX-I, an Asp49 phospholipase A₂, was purified from Bothrops moojeni venom after only one chromatographic step using reverse-phase HPLC on μ-Bondapak C-18 column. A molecular mass of 14238.71 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues.