Intrahepatic hepatitis C virus RNA quantification in microdissected hepatocytes
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- 作者：Vona ; Giovanna ; Tuveri ; Rosella ; Delpuech ; Oona ; Vallet ; Anais ; Canioni ; Danielle ; Ballardini ; Giorgio ; et. al.
- 关键词：Hepatocytes ; Intrahepatic HCV ; Hepatitis C virus ; Laser microdissection
- 刊名：Journal of Hepatology
- 出版年：2004
- 出版时间：April, 2004
- 年：2004
- 卷：40
- 期：4
- 页码：682-688
- 全文大小：1.12 M
文摘
Background/Aims: Debate continues on whether serum and intrahepatic HCV viral loads are correlated and if HCV viral load correlates with the severity of liver disease. These difficulties may at least in part be linked to liver cell heterogeneity, when total liver extracts from HCV-infected individuals are tested for HCV RNA quantification. We have therefore investigated the feasibility of quantifying HCV replication using a laser-based microdissection technique.