文摘
Visualization of A. fumigatus biofilms is influenced by SEM processing techniques. Hyphae and ECM are sensitive to interface, reagents, fixation times and drying. Shortened primary fixation, with OsO4 staining and CPD, optimally preserves structure. Contrasting with OsO4, RuO4 and Ruthenium Red enhance resolution for VP-SEM. Ionic Liquid treatment renders biofilms hydrated and conductive for SEM analysis.