Protective effect of the apoptosis-sensing nanoparticle AnxCLIO-Cy5.5

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• 作者：Howard H. Chen ; PhD^a ; ^b ; Yan Feng ; MD ; PhD^c ; Ming Zhang ; MD^c ; Wei Chao ; MD ; PhD^c ; Lee Josephson ; PhD^b ; ^e ; Stanley Y. Shaw ; MD ; PhD^a ; ^d ; David E. Sosnovik ; MD^a ; ^b ; ^{sosnovik@nmr.mgh.harvard.edu}
• 关键词：Apoptosis ; Magnetic nanoparticle ; Cardioprotection ; Iron oxide ; Gene expression
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：8
• 期：3
• 页码：291-298
• 全文大小：1252 K

文摘

The diagnostic utility of the apoptosis-sensing nanoparticle (NP), AnxCLIO-Cy5.5, is well established. Here we sought to define the pathophysiological impact of the nanoparticle (NP) on apoptotic cells. Confocal microscopy showed that AnxCLIO-Cy5.5 remained bound to apoptotic cell membranes for 3 hours but by 7 hours had become completely internalized. AnxCLIO-Cy5.5 exposure did not impact energetics, metabolism or caspase-3 activity in apoptotic cells. Gene expression in cells exposed to AnxCLIO-Cy5.5 did not reveal upregulation of pro-inflammatory or cell-death pathways. Moreover, exposure to AnxCLIO-Cy5.5 decreased the frequency of membrane rupture of early apoptotic cells. Similarly, in mice exposed to 1 hour of ischemia -reperfusion, the injection of AnxCLIO-Cy5.5 at the onset of reperfusion reduced infarct size/area at risk by 16.2 % . Our findings suggest that AnxCLIO-Cy5.5 may protect apoptotic cells by stabilizing their cell membranes and has the potential to become a theranostic agent, capable of both identifying and salvaging early apoptotic cells.

From the Clinical Editor

This study demonstrates that AnxCLIO-Cy5.5 nanoparticles may protect apoptotic cells by cell membrane stabilization and have the potential to become a ¡°theranostic agent?capable of identifying and salvaging early apoptotic cells.