Apolipoprotein A-I and Paraoxonase-1 Are Potential Blood Biomarkers for Ischemic Stroke Diagnosis

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• 作者：Kyle B. Walsh ; MD^* ; ^&dagger ; ; ^{walshk4@ucmail.uc.edu" class="auth_mail" title="E-mail the corresponding author} ; Kimberly Hart ; MA^&dagger ; ; Susan Roll ; RN^&dagger ; ; Matthew Sperling^&dagger ; ; Dusten Unruh ; PhD^&Dagger ; ; W. Sean Davidson ; PhD^§ ; ; Christopher J. Lindsell ; PhD^&dagger ; ; Opeolu Adeoye ; MD^* ; ^&dagger ; ; ^‖
• 关键词：Ischemic stroke ; intracerebral hemorrhage ; biomarker ; diagnosis ; proteomics ; plasma
• 刊名：Journal of Stroke and Cerebrovascular Diseases
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：25
• 期：6
• 页码：1360-1365
• 全文大小：558 K

文摘

Blood biomarkers for ischemic and hemorrhagic stroke diagnosis remain elusive. Recent investigations suggested that apolipoprotein (Apo), matrix metalloproteinase (MMP), and paraoxonase-1 may be associated with stroke. We hypothesized that Apo A-I, Apo C-I, Apo C-III, MMP-3, MMP-9, and paraoxonase-1 are differentially expressed in ischemic stroke, hemorrhagic stroke, and controls.

Methods

In a single-center prospective observational study, consecutive stroke cases were enrolled if blood samples were obtainable within 12 hours of symptom onset. Age- (±5 years), race-, and sex-matched controls were recruited. Multiplex assays were used to measure protein levels. The Wilcoxon signed-rank test and the Mann–Whitney U-test were used to compare biomarker values between ischemic stroke patients and controls, hemorrhagic stroke patients and controls, and ischemic and hemorrhagic stroke patients. The 95% confidence intervals (CIs) for the difference of 2 medians were calculated.

Results

Fourteen ischemic stroke case–control pairs and 23 intracerebral hemorrhage (ICH) case–control pairs were enrolled. Median Apo A-I levels were lower in ischemic stroke cases versus controls (140 mg/dL versus 175 mg/dL, difference of 35 mg/dL, 95% CI −54 to −16) and in ischemic stroke versus ICH cases (140 mg/dL versus 180 mg/dL, difference of 40 mg/dL, 95% CI −57 to −23). Median paraoxonase-1 was lower in ischemic stroke cases than in both ICH cases and matched controls. Median Apo C-I was slightly lower in ischemic stroke cases than in ICH cases. There were no differences between groups for MMP-3, MMP-9, and Apo C-III.

Conclusion

Apo A-I and paraoxonase-1 levels may be clinically useful for ischemic stroke diagnosis and for differentiating between ischemic and hemorrhagic strokes.