Calcium and magnesium elimination enhances accumulation of cardenolides in callus cultures of endemic Digitalis species of Turkey

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• 作者：G. Sahin ; S.K. Verma ; E. Gurel
• 关键词：Calcium ; Magnesium ; Cardenolides accumulation ; Digitalis cariensis Boiss. ex Jaub. et Spach ; Digitalis davisiana Heywood ; Digitalis lamarckii Ivanina ; Digitalis trojana Ivanina
• 刊名：Plant Physiology and Biochemistry
• 出版年：December, 2013
• 年：2013
• 卷：73
• 期：Complete
• 页码：139-143
• 全文大小：707 K

文摘

Elimination of calcium (Ca), magnesium (Mg) or both from the medium of callus cultures of Digitalis davisiana Heywood, Digitalis lamarckii Ivanina, Digitalis trojana Ivanina and Digitalis cariensis Boiss. ex Jaub. et Spach increased cardenolides production. Callus was induced from hypocotyl segments from one-month old seedlings were cultured on MS medium containing 0.5聽渭g聽ml^鈭? thidiazuron (TDZ) and 0.25聽渭g聽ml^鈭? indole acetic acid (IAA). After 30 days of culture, callus was transferred in hormone-free MS medium (MSO) as well as Ca or Mg or both were completely eliminated from same medium. The amount of five cardenolides from D.聽davisiana Heywood, D.聽lamarckii Ivanina, D.聽trojana Ivanina and D.聽cariensis Boiss. ex Jaub. et Spach were compared. Higher amounts of five cardenolides and total cardenolides were聽obtained when callus of four Digitalis species were incubated on MS medium lacking both聽Ca聽and聽Mg. The聽mean contents of total cardenolides obtained were in the order of D.聽lamarckii聽(2017.97聽渭g聽g^鈭?)聽>聽D.聽trojana (1385.75聽渭g聽g^鈭?)聽>聽D.聽cariensis (1038.65聽渭g聽g^鈭?)聽>聽D.聽davisiana (899.86聽渭g聽g^鈭?) when both Ca and Mg were eliminated from the medium, respectively. This protocol is useful for development of new strategies for the large-scale production of cardenolides.