Cloning and characterization of an S-RNase gene in Camellia sinensis
详细信息 查看全文
- 作者：Cheng-Cai Zhanga ; b ; c ; Li-Qiang Tana ; b ; d ; Li-Yuan Wanga ; b ; Kang Weia ; b ; Li-Yun Wua ; b ; Fen Zhanga ; b ; Hao Chenga ; b ; chenghao@tricaas.com" class="auth_mail" title="E-mail the corresponding author ; De-Jiang Nic ; nidj@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：SI ; self-incompatibility ; SSI ; sporophytic self-incompatibility ; GSI ; gametophytic self-incompatibility ; SFB/SLF ; S locus F-box ; CsS-RNase ; Camellia sinensis S-RNase ; SP ; self-pollination ; CP ; cross-pollination ; EB ; ethidium bromide ; ORF ; open reading frame ; SNP ; single nucleotide polymorphism ; dCAPS ; derived cleaved amplified polymorphic sequence ; RT-PCR ; semi-quantitative real-time PCR ; SSR ; simple sequence repeat ; SRK ; S-receptor kinase ; SCR ; S-locus cysteine-rich protein ; CAS ; conserved amin
- 刊名：Scientia Horticulturae
- 出版年：2016
- 出版时间：5 August 2016
- 年：2016
- 卷：207
- 期：Complete
- 页码：218-224
- 全文大小：2844 K
文摘
- •
A putative CsS-RNase gene (KU852488) was cloned and characterized in Camellia sinensis.
- •
The CsS-RNase is primarily expressed in the styles.
- •
The expression of CsS-RNase in self-pollinated styles was up-regulated earlier than the crossed treatments.
- •
The amino acid sequences of CsS-RNase were polymorphic in different tea cultivars.
- •
The CsS-RNase was mapped on a genetic linkage map of C. sinensis.