Concerns in the application of fluorescent probes DCDHF-DA, DHR 123 and DHE to measure reactive oxygen species in vitro
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- 作者：Mazyar Yazdani mazyar.yazdani.edu@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Reactive oxygen species (ROS) ; Oxidative stress ; Fluorescence probes ; DCDHF-DA ; DHR 123 ; DHE
- 刊名：Toxicology in Vitro
- 出版年：2015
- 出版时间：25 December 2015
- 年：2015
- 卷：30
- 期：1
- 页码：578-582
- 全文大小：390 K
文摘
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Fluorometry is among the preferred techniques for monitoring ROS in vitro.
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Three frequently used fluorescent probes are DCDHF-DA, DHR 123 and DHE.
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To obtain better results, it is crucial to consider modifications to protocols.
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Cell culture conditions and characteristics of these probes were considered here.