The suppressive effects of metformin on inflammatory response of otitis media model in human middle ear epithelial cells

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• 作者：Jae Gu Cho ; Jae Jun Song ; June Choi ; Gi Jung Im ; Hak Hyun Jung ; Sung Won Chae ; ^{koreaschae@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Metformin ; Otitis media ; LPS ; Human middle ear epitheilial cells
• 刊名：International Journal of Pediatric Otorhinolaryngology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：89
• 期：Complete
• 页码：28-32
• 全文大小：458 K

文摘

Metformin is a well-known anti-diabetic agent, but its mechanism is unclear. Recently, many reports have described the anti-inflammatory effects of metformin on various cell types, including human vascular smooth muscle cells and endothelial cells. This study was designed to investigate the anti-inflammatory effect of metformin on lipopolysaccharide (LPS) induced inflammation in human middle ear epithelial cell lines (HMEECs).

Methods

The effect of pretreatment by metformin (0, 1, 2, 4 mM) was evaluated by the inflammatory response in the HMEECs exposed to LPS (10 ng/ml). For verifying the suppression effect of metformin on the inflammatory cytokines, tumor necrosis factor-alpha (TNF-α) was evaluated by real-time polymerase chain reaction, and COX-2 protein was assessed by western blotting. Intracellular reactive oxygen species (ROS) was measured using 2′, 7′-dichlorofluorescein diacetate (DCFHDA) fluorocytometer.

Results

Stimulation by LPS 10 ng/ml concentration showed 12.4 folds increase the expression of TNF-α mRNA compared to control on HMEECs. Pretreatment of metformin dose dependently suppressed the expression of TNF-α mRNA induced by LPS (2 mM, p = 0.03). The amount of COX-2 protein production was significantly decreased by metformin pretreatment (4 mM, p = 0.01). The production of ROS was decreased significantly by pretreatment of metformin (p = 0.03).

Conclusions

These findings suggest that the inflammatory response and oxidative stress induced by LPS could be suppressed by metformin in HMEECs. Therefore, metformin may have a therapeutic potential for the treatment of the otitis media.