Wac?aw Szybalski's contribution to immunotherapy: HGPRT mutation & HAT selection as first steps to gene therapy and hybrid techniques in mammalian cells

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• 作者：Jacek J. Bigda ; Patrycja Kosza?ka
• 关键词：ADA-SCID ; adenosine deaminase-deficient severe combined immunodeficiency ; DNAse ; deoxyribonuclease ; ES cells ; embryonic stem cells ; HAT ; hypoxanthine ; aminopterin ; thymidine ; HPRT ; hypoxanthine-guanine phosphoribosyl transferase ; IMP ; inosine monophosphat
• 刊名：Gene
• 出版年：2013
• 出版时间：10 August, 2013
• 年：2013
• 卷：525
• 期：2
• 页码：158-161
• 全文大小：168 K

文摘

In this report we describe Wac?aw Szybalski's fundamental contribution to gene therapy and immunotherapy. His 1962 PNAS paper (Szybalska and Szybalski, 1962) documented the first successful gene repair in mammalian cells. Furthermore, this was also the first report on the HAT selection method used later in many applications. Most importantly, somatic cell fusion and HAT selection were subsequently used to develop monoclonal antibody technology, which contributed significantly to the progress of today's medicine.