Built a UHPLC–MS/MS method for simultaneous quantification of Sabarubicin and its alcohol metabolite in human plasma.
Lower limit of quantitation was 2 ng/mL for Sabarubicin and 0.5 ng/mL for M3 with a sample volume of 100 μL.
Method was proved to be sufficient for pharmacokinetics study in Chinese SCLC patients.