Problems involving the determination of the column-only band broadening in columns producing narrow and tailed peaks
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- 作者：Yoachim Vanderheyden ; Kim Vanderlinden ; Ken Broeckhoven ; Gert Desmet ; gedesmet@vub.ac.be" class="auth_mail" title="E-mail the corresponding author
- 关键词：Extra-column band broadening ; Statistical peak moments ; Deconvolution ; Peak width
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1440
- 期：Complete
- 页码：74-84
- 全文大小：1731 K
文摘
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Peak variance read-out methods (peak width, moments, deconvolution) were compared.
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Extra-column band broadening effects are accurately measured using moment integration.
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Peak width methods incorrectly estimate the variance of narrow and tailed peaks.
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Deconvolution can filter away extra-column contributions from the entire peak shape.
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Half height, 4σ and moment methods can predict performance at other column lengths