Effects of major nutrients, growth regulators and inoculum size on enhanced growth and camptothecin production in adventitious root cultures of Ophiorrhiza mungos L.
Adventitious roots were induced from Ophiorrhiza mungos aseptic leaf explants. Established isolated root cultures of Ophiorrhiza mungos. Culture parameters were optimized and proposed a modified nutrient medium (MS1). A 16.65 fold increase in fresh growth index was obtained in the MS1 medium. A 3.44 fold increase in CPT accumulation was achieved in the MS1 medium.