Secretory pathway Ca2+-ATPase isoform 1 knockdown promotes Golgi apparatus stress injury in a mouse model of focal cerebral ischemia-reperfusion: In vivo and in vitro study
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- 作者：Yongmei Fana ; Changjie Zhanga ; Wenna Penga ; Ting Lib ; Jing Yina ; Ying Konga ; Chunna Lana ; Xiaofang Lia ; Rumi Wanga ; Zhiping Hub ; huzhiping2014@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：SPCA1 ; Cerebral ischemia&ndash ; reperfusion ; Golgi apparatus stress ; MCAO/R ; OGD/R ; Nitric oxide
- 刊名：Brain Research
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：1642
- 期：Complete
- 页码：189-196
- 全文大小：2345 K
文摘
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Two hours of ischemia then reperfusion induced SPCA1 level decrease and oxidative stress.
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SPCA1 lentivirus achieve knockdown of SPCA1 gene, SPCA1 decreased.
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SPCA1 knockdown increase infarction volume, neurological defects.
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SPCA1 knockdown facilitate peroxidative production.
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SPCA1 knockdown increased H2O2-induced LDH, and elevated caspase3 level in N2a cells.