Cellular uptake of poly(allylamine hydrochloride) microcapsules with different deformability and its influence on cell functions

详细信息    查看全文

• 作者：Wei Yu ; Wenbo Zhang ; Ying Chen ; Xiaoxue Song ; Weijun Tong ; Zhengwei Mao ; ^{zwmao@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Changyou Gao ; ^{cygao@mail.hz.zj" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Poly(allylamine hydrochloride) ; Microcapsules ; Deformability ; Cell uptake ; Cell functions
• 刊名：Journal of Colloid and Interface Science
• 出版年：2016
• 出版时间：1 March 2016
• 年：2016
• 卷：465
• 期：Complete
• 页码：149-157
• 全文大小：2033 K

文摘

It is important to understand the safety issue and cell interaction pattern of polyelectrolyte microcapsules with different deformability before their use in biomedical applications. In this study, SiO₂, poly(sodium-p-styrenesulfonate) (PSS) doped CaCO₃ and porous CaCO₃ spheres_, all about 4 μm in diameter, were used as templates to prepare microcapsules with different inner structure and subsequent deformability. As a result, three kinds of covalently assembled poly(allylaminehydrochloride)/glutaraldehyde (PAH/GA) microcapsules with similar size but different deformability under external osmotic pressure were prepared. The impact of different microcapsules on cell viability and functions are studied using smooth muscle cells (SMCs), endothelial cells (ECs) and HepG2 cells. The results demonstrated that viabilities of SMCs, ECs and HepG2 cells were not significantly influenced by either of the three kinds of microcapsules. However, the adhesion ability of SMCs and ECs as well as the mobility of SMCs, ECs and HepG2 cells were significantly impaired after treatment with microcapsules in a deformability dependent manner, especially the microcapsules with lower deformability caused higher impairment on cell functions. The cellular uptake kinetics, uptake pathways, intracellular distribution of microcapsules are further investigated in SMCs to reveal the potential mechanism. The SMCs showed faster uptake rate and exocytosis rate of microcapsules with lower deformability (Cap@CaCO₃/PSS and Cap@CaCO₃), leading to higher intracellular accumulation of microcapsules with lower deformability and possibly larger retardation of cell functions. The results pointed out that the deformability of microcapsules is an important factor governing the biological performance of microcapsules, which requires careful adjustment for further biomedical applications.