Degradome sequencing reveals an endogenous microRNA target in C. elegans

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• 作者：June Hyun Park^a ; Soungyub Ahn^c ; Soyoung Kim^a ; Junho Lee^d ; Jin-Wu Nam^b ; ^{jwnam@hanyang.ac.kr} ; Chanseok Shin^a ; ^{cshin@snu.ac.kr}
• 关键词：MicroRNA ; Degradome sequencing ; MicroRNA-directed cleavage ; C. elegans
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：2 April, 2013
• 年：2013
• 卷：587
• 期：7
• 页码：964-969
• 全文大小：432 K

文摘

Caenorhabditis elegans microRNAs (miRNAs) bind to partially complementary sequences in the 3¡ä untranslated region of target mRNAs, resulting in translational repression through mRNA destabilization. High-throughput sequencing of RNA cleavage fragments was performed to directly detect miRNA-directed cleavage targets in adult stage C. elegans. From this analysis, we found that miR-249 directed the cleavage of the ZK637.6 transcript with extensive and evolutionarily conserved complementarity in nematode. In addition, expression of the ZK637.6 transcript was strongly dependent on the expression of miR-249. These findings may lead to a better understanding of miRNA-mediated gene regulation in nematodes.