Yap1: A DNA damage responder in Saccharomyces cerevisiae

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• 作者：Lori A. Rowe^a ; ^b ; Natalya Degtyareva^a ; ^c ; Paul W. Doetsch^a ; ^c ; ^d ; ^e ; ^{medpwd@emory.edu}
• 关键词：DNA damage signaling ; Oxidative stress ; Base excision repair ; Reactive oxygen species ; Genome instability
• 刊名：Mechanisms of Ageing and Development
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：133
• 期：4
• 页码：147-156
• 全文大小：1140 K

文摘

Activation of signaling pathways in response to genotoxic stress is crucial for cells to properly repair DNA damage. In response to DNA damage, intracellular levels of reactive oxygen species increase. One important function of such a response could be to initiate signal transduction processes. We have employed the model eukaryote Saccharomyces cerevisiae to delineate DNA damage sensing mechanisms. We report a novel, unanticipated role for the transcription factor Yap1 as a DNA damage responder, providing direct evidence that reactive oxygen species are an important component of the DNA damage signaling process. Our findings reveal an epistatic link between Yap1 and the DNA base excision repair pathway. Corruption of the Yap1-mediated DNA damage response influences cell survival and genomic stability in response to exposure to genotoxic agents.