MSAP marker based DNA methylation study in Salicornia brachiata DREB2A transgenic tobacco

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• 作者：Prashant More^a ; ^b ; Parinita Agarwal^a ; Shaik G. Mastan^a ; ^b ; ¹ ; Pradeep K. Agarwal^a ; ^b ; ^{pagarwal@csmcri.org" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AFLP ; Amplified fragment length polymorphism ; DREB ; Dehydration responsive element binding ; DREs ; Drought responsive elements ; HSF ; Heat shock transcription factor ; HSP ; Heat shock protein ; MP ; Methylation polymorphic ; MSAP ; Methylation sensitive amplified polymorphism ; TF ; Transcription factor ; VA ; Vector alone ; WT ; Wild type
• 刊名：Plant Gene
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：6
• 期：Complete
• 页码：77-81
• 全文大小：578 K

文摘

DNA methylation plays an important role in gene regulation during growth, development and different stresses. We have studied the effect of transgene induced DNA methylation pattern in SbDREB2A overexpressed tobacco transgenic lines. DREB (Dehydration-responsive element binding) transcription factor (TF) plays a vital role in providing stress tolerance in ABA-independent manner by regulating downstream genes in plants. An increase in percent methylation was observed in the transgenic lines as compared to wild type (WT) and plants transformed with vector alone (VA). Among the three transgenic lines, S-201 showed maximum methylation and methylation polymorphism, whereas, the demethylation pattern was observed almost similar for all the transgenic lines. This indicates that DREB gene might be involved during methylation events in transgenic plants by regulating certain regions of the plant genome that plays important role during stress conditions.