Differential cytotoxic effects of 7-dehydrocholesterol-derived oxysterols on cultured retina-derived cells: Dependence on sterol structure, cell type, and density
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- 作者：Bruce A. Pfeffera ; b ; c ; Libin Xud ; Ned A. Portere ; Sriganesh Ramachandra Raoa ; b ; c ; Steven J. Flieslera ; b ; c ; fliesler@buffalo.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Photoreceptor ; Mü ; ller cell ; Retinal pigment epithelium ; Oxysterol ; Cell viability assay ; Cell culture
- 刊名：Experimental Eye Research
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：145
- 期：Complete
- 页码：297-316
- 全文大小：4277 K
文摘
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Four oxysterols had a range of effects on viability of cultured retinal cell types.
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A 7-dehydrocholesterol-derived oxysterol endoperoxide (EPCD) was most potent.
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661W, a murine cone photoreceptor surrogate, was most sensitive to oxysterols.
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Results correlated well with photoreceptor death in a rat model of human disease.
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In vitro model may be useful to explore molecular mechanisms of human disease.