Mutation of heme c axial ligands in d-fructose dehydrogenase for investigation of electron transfer pathways and reduction of overpotential in direct electron transfer-type bioelectrocatalysis
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- 作者：Yuya Hibino ; Shota Kawai ; Yuki Kitazumi ; Osamu Shirai ; Kenji Kano ; kano.kenji.5z@kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Fructose dehydrogenase ; Direct electron transfer ; Electron transfer pathway ; Overpotential ; Axial ligand ; Heme c
- 刊名：Electrochemistry Communications
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：67
- 期：Complete
- 页码：43-46
- 全文大小：380 K
文摘
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Bioelectrocatalytic measurements were coupled with a protein engineering technique.
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Mutations were introduced into the axial ligand of one of the three hemes.
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M450Q mutant reduced the overpotential of catalytic d-fructose oxidation.
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M301Q mutant remained almost unchanged.
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M578Q mutant drastically decreased DET-type catalytic activity.