Quantifying NAD(P)H production in the upper Entner-Doudoroff pathway from Pseudomonas putida KT2440
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- 作者：Karel Olavarria ; kolavarria@usp.br" class="auth_mail" title="E-mail the corresponding author ; Marina Pupke Marone ; Henrique da Costa Oliveira ; Juan Camilo Roncallo ; Fernanda Nogales da Costa Vasconcelos ; Luiziana Ferreira da Silva ; José ; Gregó ; rio Cabrera Gomez
- 关键词：G6P ; glucose-6-phosphate ; G6PDH ; glucose-6-phosphate dehydrogenase ; PputG6PDH-1 ; glucose-6-phosphate dehydrogenase encoded by the gene zwf-1 from Pseudomonas putida KT2440 ; GND ; 6-phospho-gluconate dehydrogenase ; EDP ; Entner&ndash ; Doudoroff pathway
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：908-915
- 全文大小：1278 K
文摘
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The first kinetic characterization of PputG6PDH-1 is presented.
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The relative production of NADH and NADPH by PputG6PDH-1 is quantified.
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The stoichiometric matrix of in silico metabolic models for Pseudomonas putida must be modified.