Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003
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- 作者：Huan Zhang ; Lulu Shi ; Xinlei Mao ; Jinping Lin ; jplin@ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Dongzhi Wei
- 关键词：mADH ; membrane-bound alcohol dehydrogenase ; mALDH ; membrane-bound aldehyde dehydrogenase ; mGLDH ; membrane-bound sorbitol dehydrogenase ; mGDH ; membrane-bound glucose dehydrogenase ; GA-2-DH ; membrane-bound 2-keto-d-gluconate dehydrogenase ; DCW ; dry cell weight ; OUR ; oxygen uptake rate ; DO ; dissolved oxygen ; qRT-PCR ; quantitative real-time polymerase chain reaction ; HPLC ; high-performance liquid chromatography
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 November 2016
- 年：2016
- 卷：237
- 期：Complete
- 页码：18-24
- 全文大小：1195 K
文摘
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Overexpression of membrane-bound alcohol dehydrogenase (mADH) accelerated cell growth.
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Overexpression of mADH induced the transcriptional levels of respiratory chain-related enzyme genes.
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The oxygen uptake rate and membrane-bound aldehyde dehydrogenase activity was increased in the engineered strain.
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The engineered strain can efficiently increase glycolic acid production.