GADH screen-printed biosensor for gluconic acid determination in wine samples
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- 作者：Lorena del Torno-de Rom谩n ; M. Asunci贸n Alonso-Lomillo ; Olga Dom铆nguez-Renedo ; Arrate Jaureguibeitia ; M. Julia Arcos-Mart铆nez
- 关键词：Screen-printed carbon electrodes ; Chronoamperometric biosensor ; Gluconate dehydrogenase ; Tetrathiafulvalene ; Gluconic acid
- 刊名：Sensors and Actuators B: Chemical
- 出版年：1 March, 2014
- 年：2014
- 卷：192
- 期：Complete
- 页码：56-59
- 全文大小：844 K
文摘
Gluconate dehydrogenase (GADH) biosensors have been developed for the satisfactory determination of gluconic acid in wine samples, without any pretreatment. The biosensors have been fabricated by cross-linking immobilization of GADH onto screen-printed carbon electrodes, containing the mediator tetrathiafulvalene (TTF). Chronoamperograms have been registered at +100 mV vs. screen-printed Ag/AgCl electrode by successive additions of a gluconic acid solution in the concentration range from 9.0 to 131.4 渭M. This method shows a reproducibility of 8.1% (n = 3) related to the slopes of these calibration curves and a repeatability of 3.2% (n = 5). The procedure has shown an average capability of detection of 9.0 渭M for a probability of false positive and negative of 0.05. Wine samples have been analyzed with these biosensors, obtaining satisfactory results.