Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and sufficient donors

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• 作者：Vassilis L. Tzounakas^a ; Anastasios G. Kriebardis^b ; Hara T. Georgatzakou^a ; Leontini E. Foudoulaki-Paparizos^c ; ¹ ; Monika Dzieciatkowska^d ; Matthew J. Wither^d ; Travis Nemkov^d ; Kirk C. Hansen^d ; Issidora S. Papassideri^a ; Angelo D׳Alessandro^d ; ^{angelo.dalessandro@ucdenver.edu" class="auth_mail" title="E-mail the corresponding author} ; Marianna H. Antonelou^a ; ^{manton@biol.uoa.gr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AnnV ; annexin V ; CPD ; citrate-phosphate-dextrose ; FRAP ; ferric reducing antioxidant power ; FSC ; forward scatter ; G6PD ; glucose-6-phosphate dehydrogenase ; G6PD&minus ; G6PD deficiency ; Hb ; hemoglobin ; Hct ; hematocrit ; K+ ; potassium ; MCF ; mean corpuscular fragility ; MFI ; mechanical fragility index ; MP ; micoparticles ; microvesicles ; MPPA ; microparticles pro-coagulant activity ; NAC ; N-acetylcysteine ; NS ; non-stored ; PBS ; phosphate buffer saline ; PCI ; protein carbonylation index ; PS ; phosphatidylseri
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：618-627
• 全文大小：1178 K

文摘

This article contains data on the variation in several physiological parameters of red blood cells (RBCs) donated by eligible glucose-6-phosphate dehydrogenase (G6PD) deficient donors during storage in standard blood bank conditions compared to control, G6PD sufficient (G6PD⁺) cells. Intracellular reactive oxygen species (ROS) generation, cell fragility and membrane exovesiculation were measured in RBCs throughout the storage period, with or without stimulation by oxidants, supplementation of N-acetylcysteine and energy depletion, following incubation of stored cells for 24 h at 37 °C. Apart from cell characteristics, the total or uric acid-dependent antioxidant capacity of the supernatant in addition to extracellular potassium concentration was determined in RBC units. Finally, procoagulant activity and protein carbonylation levels were measured in the microparticles population. Further information can be found in “Glucose 6-phosphate dehydrogenase deficient subjects may be better “storers” than donors of red blood cells” [1].