Purification of a dimeric arginine deiminase from Enterococcus faecium GR7 and study of its anti-cancerous activity
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- 作者：Baljinder Kaur ; baljinderbt@gmail.com" class="auth_mail" title="E-mail the corresponding author ; baljinderbt@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Rajinder Kaur
- 关键词：ADI ; arginine deiminase ; ASS ; argininosuccinate synthetase ; CK ; carbamate kinase ; CF ; cytoplasmic fractions ; DMSO ; dimethylsulfoxide ; GRAS ; generally recognized as safe ; HKC ; heat-killed cells ; Hep-G2 ; Human hepatocellular carcinoma cell line ; IC50 ; half-maximal inhibitory concentration ; LAB ; lactic acid bacteria ; MTT ; 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; MCF7 ATCC-HTB-22 ; Human Breast carcinoma cell line ; OTC ; ornithine transcarbamylase ; OCT ; ornithine carbamoyltransfer
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：53-60
- 全文大小：904 K
文摘
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E. faecium GR7 isolated from dairy a product was used as a source of ADI enzyme.
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A three-step protocol for purification of ADI to homogeneity from E. faecium GR7.
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Purified enzyme showed the specific ADI activity of 76.65 IU/mg with a 49.17% recovery.
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First time reported heterodimer ADI protein with a molecular weight of 94.36 kDa.
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Purified ADI exhibited growth inhibition at IC50-1.95 μg/ml against Hep-G2 cells.