Cloning the putative gene of vinyl phenol reductase of Dekkera bruxellensis in Saccharomyces cerevisiae

详细信息    查看全文

• 作者：Diego Romano¹ ; Federica Valdetara¹ ; Paolo Zambelli ; Silvia Galafassi ; Valerio De Vitis ; Francesco Molinari ; Concetta Compagno ; Roberto Foschino ; Ileana Vigentini ; ^{ileana.vigentini@unimi.it}
• 关键词：Wine ; B. bruxellensis ; Volatile phenols ; VPR enzyme ; Genetic polymorphism
• 刊名：Food Microbiology
• 出版年：2017
• 出版时间：May 2017
• 年：2017
• 卷：63
• 期：Complete
• 页码：92-100
• 全文大小：1041 K
• 卷排序：63

文摘

VPR enzyme of B. bruxellensis has been identified through the expression of a functional protein in S. cerevisiae. The Rossmann fold-like region of VPR, involved in the reductase activity, is a conserved domain in D. bruxellensis. No direct correlation exists between the genetic polymorphism of VPR gene and the production of volatile phenols. VPR is a SOD; this evidence is particularly interesting in terms of adaptive evolution of the species to wine environment.