A two component system is involved in acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus

详细信息    查看全文

• 作者：Yanhua Cui^a ; ^{yhcui@hit.edu.cn} ; Wei Liu^a ; Xiaojun Qu^b ; Zhangting Chen^a ; Xu Zhang^a ; Tong Liu^a ; Lanwei Zhang^a
• 关键词：Acid adaptation ; Two component system ; Histidine protein kinase ; Response regulator ; Lactobacillus delbrueckii subsp. bulgaricus
• 刊名：Microbiological Research
• 出版年：2012
• 出版时间：20 May, 2012
• 年：2012
• 卷：167
• 期：5
• 页码：253-261
• 全文大小：973 K

文摘

The Gram-positive bacterium Lactobacillus delbrueckii subsp. bulgaricus is of vital importance to the food industry, especially to the dairy industry. Two component systems (TCSs) are one of the most important mechanisms for environmental sensing and signal transduction in the majority of Gram-positive and Gram-negative bacteria. A typical TCS consists of a histidine protein kinase (HPK) and a cytoplasmic response regulator (RR). To investigate the functions of TCSs during acid adaptation in L. bulgaricus, we used quantitative PCR to reveal how TCSs expression changes during acid adaptation. Two TCSs (JN675228/JN675229 and JN675230/JN675231) and two HPKs (JN675236 and JN675240) were induced during acid adaptation. These TCSs were speculated to be related with the acid adaptation ability of L. bulgaricus. The mutants of JN675228/JN675229 were constructed in order to investigate the functions of JN675228/JN675229. The mutants showed reduced acid adaptation compared to that of wild type, and the complemented strains were similar to the wild-type strain. These observations suggested that JN675228 and JN675229 were involved in acid adaptation in L. bulgaricus. The interaction between JN675228 and JN675229 was identified by means of yeast two-hybrid system. The results indicated there is interaction between JN675228 and JN675229.