MIP-graphene-modified glassy carbon electrode for the determination of trimethoprim

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• 作者：H茅lder da Silva ; Jo茫o G. Pacheco ; J煤lia MCS Magalh茫es ; Subramanian Viswanathan ; Cristina Delerue-Matos
• 关键词：Trimethoprim ; Electrochemical sensor ; Molecularly Imprinting Polymer (MIP) ; Graphene ; Pyrrole
• 刊名：Biosensors and Bioelectronics
• 出版年：15 February, 2014
• 年：2014
• 卷：52
• 期：Complete
• 页码：56-61
• 全文大小：724 K

文摘

A novel sensitive electrochemical sensor was developed by electropolymerization of pyrrole (PY) and molecularly imprinted polymer (MIP) which was synthesized onto a glassy carbon electrode (GCE) in aqueous solution using cyclic voltammetry in the presence of Trimethoprim (TMP) as template molecules. Furthermore, a previous electrode modification was performed by deposition of a suspension of graphene on the electrode's surface. The performance of the imprinted and non-imprinted (NIP) films was evaluated by impedance spectroscopy (EIS) and cyclic voltammetry (CV) of a ferric solution. The molecularly imprinted film exhibited a high selectivity and sensitivity toward TMP. The sensor presented a linear range, between peak current intensity and logarithm of TMP concentration between 1.0脳10^鈭? and 1.0脳10^鈭? M. The results were accurate (with recoveries higher than 94%), precise (with standard deviations less than 5%) and the detection limit was 1.3脳10^鈭? M. The new sensor is selective, simple to construct and easy to operate. The MIP sensor was successfully applied to quantify TMP in urine samples.