A facile and efficient transposon mutagenesis method for generation of multi-codon deletions in protein sequences
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- 作者：Shu-su Liua ; liushs@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xuan Weia ; weixuan@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Qun Jia ; jiqun@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiu Xina ; xinxiu@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Biao Jianga ; jiangbiao@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jia Liua ; b ; liujia@shanghaitech.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Asymmetric transposon ; Codon deletions ; Mutant library ; Green fluorescent protein ; Reading frame selection
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：27-34
- 全文大小：1706 K
文摘
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Codon deletion mutagenesis (CDM) relies on an improved asymmetric Mu transposon.
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CDM can generate up to five consecutive, in-frame codon deletions.
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CDM has high mutation efficiency (>90%).
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CDM requires simple DNA manipulation process.
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CDM was performed on GFP to sample its tolerance to amino acid deletions.