Leukocyte-platelet-rich plasma diminishes bone matrix deposition in rat calvaria treated with autograft due to simultaneous increase in immunohistochemical expression of Indian Hedgehog, transforming growth factor-尾, and parathyroid-1 receptor

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• 作者：Daniel Xavier Cerci ; Giovanna Schirmer Portela ; Emanuelle Juliana Cunha ; Joã ; o Ricardo de Almeida Grossi ; Joã ; o Cesar Zielak ; Melissa Rodrigues Araú ; jo ; Rafaela Scariot ; Tatiana Miranda Deliberador ; Allan Fernando Giovanini ; ^{afgiovanini@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Platelet-rich plasma ; IHH ; PTH1R ; TGF-尾 ; Bone regeneration
• 刊名：Journal of Cranio-Maxillofacial Surgery
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：43
• 期：8
• 页码：1470-1477
• 全文大小：4281 K

文摘

This study evaluated the immunohistochemical presence of Indian Hedgehog (IHH), transforming growth factor-尾 (TGF-尾), and parathyroid-1 receptor (PTH1R) in calvaria bone repair, and compared these results with the histological bone matrix features in defects treated with autograft in the presence or absence of L-PRP.

Material and methods

An artificial bone defect measuring 5 × 1 mm was produced in the calvaria of 28 Wistar rats. Randomly the defects were treated with autograft and autograft mixed with L-PRP. The animals were euthanized at 15 and 40 days post-surgery. Data were analyzed by Student–Newman–Keuls test (p ≤ .05) for immunohistochemical interpretation.

Results

The results revealed that the histological characteristic of bone matrix deposited in the defect was different in the defects treated with L-PRP. The group that received only the autograft demonstrated larger haversian bone matrix deposited, whereas the group that received autograft mixed with L-PRP revealed trabecular bone deposition. These results coincided with significantly higher immunopositivity for IHH, TGF-尾1, and PTH1R in the L-PRP group.

Conclusion

These results suggest that L-PRP altered the biological characteristic of the autograft, increasing the bone cells IHH+ but inducing a trabecular bone associated with intense quantities of TGF-尾 and PTH1R.