Variation in clinical vitamin D status by DiaSorin Liaison and LC-MS/MS in the presence of elevated 25-OH vitamin D₂

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• 作者：Lawrence de Koning^a ; ^b ; ^c ; M. Rabie Al-Turkmani^d ; Anders H. Berg^e ; Aida Shkreta^c ; Terence Law^c ; Mark D. Kellogg^c ; ^{mark.kellogg@childrens.harvard.edu}
• 关键词：25-OH ; 25 Hydroxy ; ABDV ; Accuracy Based Vitamin D Survey ; CLSI ; Clinical and Laboratory Standards Institute ; IDS ; Immunodiagnostic Systems ; DEQAS ; Vitamin D External Quality Assessment Scheme
• 刊名：Clinica Chimica Acta
• 出版年：2013
• 出版时间：16 January, 2013
• 年：2013
• 卷：415
• 期：Complete
• 页码：54-58
• 全文大小：423 K

文摘

Background

We compared total 25-OH vitamin D status measured by DiaSorin Liaison and tandem mass spectrometry (LC-MS/MS) among patients with high and low 25-OH vitamin D₂.

Methods

Total 25-OH vitamin D was measured in plasma containing high (> 25 nmol/l or > 50 % , n = 26) and low (< 2.5 nmol/l, n = 29) 25-OH vitamin D₂ using DiaSorin Liaison and an LC-MS/MS method using NIST 972-verified calibrators. Samples were classified as vitamin D adequate (total 25-OH vitamin D ¡Ý 50 nmol/l), and inadequate or deficient (< 50 nmol/l) by each method. Deming and multiple linear regression were used to compare methods.

Results

Samples were significantly more likely to be classified as inadequate or deficient by DiaSorin Liaison (36 % ) vs LC-MS/MS (9 % ). This increased in the presence of high 25-OH vitamin D2 (42 % vs 0 % ). Total 25-OH vitamin D by DiaSorin Liaison was 26.0 nmol/l lower than LC-MS/MS, which increased to 34.1 nmol/l among samples with high 25-OH vitamin D₂. This was attributed to lower recovery of 25-OH vitamin D₂ (proportional bias = 0.64 nmol/l) by DiaSorin Liaison, independent of D₃ (proportional bias = 0.86 nmol/l).

Conclusions

Patients were more likely to be classified as vitamin D inadequate or deficient by DiaSorin Liaison compared to an LC-MS/MS method, which was in part due to the presence of 25-OH vitamin D₂.