Nitric oxide synthase is up-regulated in muscle fibers in muscular dystrophy
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- 作者：Karla Punkt ; Stefan Schering ; Sabine Lö ; ffler ; Evgeny A. Minin ; Vera E. Samoilova ; Martin Hasselblatt ; Werner Paulus ; Ursula Mü ; ller-Werdan ; Uta Demus ; Gabriele Koehler et al.
- 关键词：Nitric oxide synthase ; Duchenne muscular dystrophy ; Immunohistochemistry ; Cytophotometry
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 出版时间：15 September 2006
- 年：2006
- 卷：348
- 期：1
- 页码：259-264
- 全文大小：316 K
文摘
Nitric oxide (NO) mediates fundamental physiological actions on skeletal muscle. The neuronal NO synthase isoform (NOS1) was reported to be located exclusively in the sarcolemma. Its loss from the sarcolemma was associated with development of Duchenne muscular dystrophy (DMD). However, new studies evidence that all three NOS isoforms—NOS1, NOS2, and NOS3—are co-expressed in the sarcoplasm both in normal and in DMD skeletal muscles. To address this controversy, we assayed NOS expression in DMD myofibers in situ cytophotometrically and found NOS expression in DMD myofibers up-regulated. These results support the hypothesis that NO deficiency with consequent muscle degeneration in DMD results from NO scavenging by superoxides rather than from reduced NOS expression.