Magnetic resonance imaging of Fe₃O₄@SiO₂-labeled human mesenchymal stem cells in mice at 11.7?T

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• 作者：Peiwei Yi^a ; ^c ; ¹ ; Guangcun Chen^b ; ¹ ; Hailu Zhang^a ; Fei Tian^b ; Bo Tan^a ; Jianwu Dai^b ; ^d ; Qiangbin Wang^b ; ^{qbwang2008@sinano.ac.cn} ; Zongwu Deng^a ; ^{zwdeng2007@sinano.ac.cn}
• 关键词：MRI ; hMSCs ; Fe3O4@SiO2 ; Single cell sensitivity
• 刊名：Biomaterials
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：34
• 期：12
• 页码：3010-3019
• 全文大小：2311 K

文摘

Fe₃O₄@SiO₂ core-shell nanoparticles were synthesized and used to label human mesenchymal stem cells (hMSCs) for in?vitro and in?vivo magnetic resonance imaging study. The diameter of the nanoparticles is 24-30?nm with a Fe₃O₄ core of ¡«8?nm and a SiO₂ shell of ¡«8?nm. Transverse relaxivity of the nanoparticles dispersed in water is measured to be ¡«106?mM^?1?s^?1. After incubation with hMSCs for 12?h at a concentration of 100?¦Ìg Fe/mL, cellular uptake of Fe₃O₄@SiO₂ is 20-100?pg Fe/cell, which are located predominantly in the cytoplasm of cells. This level of uptake exhibits no significant influence on hMSCs' viability and differentiation. In?vitro imaging of Fe₃O₄@SiO₂-labeled hMSCs evenly distributed in agarose gel yields single cell sensitivity at 11.7?T. In?vivo imaging of Fe₃O₄@SiO₂-labeled hMSCs injected into the left brain hemisphere of nude mice yields imaging sensitivity of ¡«130 hMSCs.